Sleep research article
Identification of PfSR1-MT as melatonin-responsive GPCR-like candidate from the human malaria parasite <i>Plasmodium falciparum</i>.
Authors: Singh MK , Dias BKM , Ahmed W , Dos Santos Oliveira J , Cecon E , Gbahou F , Torres TM , Guimarães E , Houzé L , Mohanty A , Nakabashi M , Vélez JAC , Giostri VEMA , Paixão MW , Masri B , Jockers R , Garcia CRS
One-line summary
A sleep science research article on Identification of PfSR1-MT as melatonin-responsive GPCR-like candidate from the human malaria parasite <i>Plasmodium falciparum</i>..
Sleep health notes
Sleep health notes will be added by the Sleepatch editorial team.
中文解读
中文解读待补充：本站会优先为失眠研究、睡眠质量改善、昼夜节律等高价值睡眠研究添加中文说明。
Original abstract
The synchronizing effect of melatonin on the malaria parasite <i>Plasmodium falciparum</i> is well documented, yet its receptor remains elusive. In mammals, melatonin signals through seven-transmembrane (7TM) G protein-coupled receptors. Here, we characterized two putative <i>Plasmodium</i> melatonin receptor candidates, PfSR1-MT and PfSR10, expressed during the asexual parasite stage. We show that PfSR1-MT, but not PfSR10, binds 2-[¹²⁵I]iodomelatonin with high affinity (pM) when heterologously expressed in human cells. Molecular modeling and mutagenesis studies identified the melatonin-binding site within the transmembrane core of PfSR1-MT. Melatonin binding promoted the extracellular domain (ECD) movement of PfSR1-MT and cytosolic Ca²⁺ mobilization. We identified ARL34, an indole compound specifically binding and activating PfSR1-MT, but not human melatonin receptors, and impairing both asexual parasite proliferation and gametocyte development, supporting its potential as a novel antimalarial targeting the PfSR1-MT signaling pathway. Together, these findings identify PfSR1-MT as a melatonin-responsive GPCR-like candidate in the human malaria parasite <i>Plasmodium falciparum</i>.
Links and sources
This content is provided for informational and educational purposes only and does not constitute medical advice, diagnosis, or treatment. Sleep disorders, chronic insomnia, sleep apnea, and other conditions must be evaluated and treated by a qualified healthcare professional. If you experience persistent or severe sleep problems, consult a licensed physician or sleep specialist. Research cited refers to peer-reviewed studies; individual results may vary. Sleepatch does not endorse any specific medication, supplement, or therapy.
Want a personalized sleep improvement plan?
Sleepatch can prepare a customized sleep wellness program, insomnia relief guide, and evidence-based sleep coaching based on your needs.
Explore sleep services
Comments